Multiplexed Assay Kits

Nirmidas Biotech Inc. is proud to announce the availability of our pGOLD multiplexed assay kits for the rapid detection (< 2 hours) of antibodies against a panel of infectious diseases. Our assay kits use a ultra-low volume of patient sample (2-5 ul) for simultaneous detection of different antibody sub types (IgG/IgM/IgA). Our assay kits are compatible with MidaScan/MidaScan-IR scanners and other existing fluorescence based microplate readers.

How it works

In each microarray module, a panel of infectious disease antigens are immobilized on pGOLD surface in triplicates. During assay procedure, test samples are incubated on the microarray, where different isotypes of human antibodies if present, will be captured by corresponding antigens. Following labeling by dye-labeled (multi-colored) capture antibodies, the microarray module is scanned with a fluorescence microplate reader. Fluorescense signal and intensity at specific antigen spots correlates to the amount of specific antibody isotypes present against the corresponding antigen.

ELISA-like assay plate consisting of 4 pGOLD slides

ELISA-like assay plate consisting of 4 pGOLD slides

Key features:

  • A novel signal amplifying platform for biological testing and diagnostics
  • Highly sensitive fluorescence enhanced multiplex assays, in ELISA-like microplate format
  • Each assay contains 4 biochips and can test up to 56 samples including controls.
  • ~4.5 logs of dynamic range in signal
  • Broad (6-7 logs) concentration range
  • Detects IgG and IgM or IgA simultaneously in each sample against multiple antigens in each panel
  • Suitable for ELISA-like assay procedure
  • Compatible with fluorescence-based whole plate reader (using existing instrument or MidaScan/MidaScan-IR scanner provided by Nirmidas)

Multiplexed Assay Kits enable:

  • Ultra-sensitive assays with ultra-low sample volume (2-5ul)
  • Analysis of different sample matrices (whole blood, plasma, saliva (100-200ul))
  • Low cost per sample per analyte

Research Use Only

 

 

Zika/DenguE and Avidity Assay Product

Nirmidas Biotech Inc. announces the availability of three pGOLD-Zika/Dengue IgG/IgM/IgA Assay kits for rapid, simultaneous, multiplexed detection of IgG, IgM and IgA antibodies against Zika virus (ZIKV) and Dengue virus (DENV), each requiring only 1 micro-liter of patient sera. A unique IgG avidity test against both ZIKV and DENV is also developed. These assays, published in the March 6 issue of Nature Medicine can facilitate differentiating ZIKV from DENV infection, and revealing whether the infection occurred recently (low avidity) or in the past (high avidity) for both Zika and Dengue infections.

The current Zika diagnostic tests are non-ideal due to narrow diagnostic window (RNA detection by PCR), cross reactivity (Zika IgM test) or time-consuming and laborious procedures (plaque reduction neutralization testing, PRNT). The simultaneous ZIKV and DENV IgG/IgA testing, enabled by the pGOLD assay, is novel with an important finding that the cross-reactivity problem between ZIKV and DENV IgG and IgA antibodies occurs at a much lower degree than IgM antibodies, thus allowing highly specific diagnosis of ZIKV infection and differentiation from Dengue infection. ZIKV IgA positivity is found to be highly specific to early stage ZIKV infection. For a ZIKV infected cohort with symptom onset to sample collection time of 2-90 days in the acute-convalescent phase, the pGOLD IgG/IgA test can diagnose ZIKV infection with > 90% sensitivity and ~ 98% specificity, with little cross-reactivity problem due to chronic or on-going DENV infection. For an acute-phase ZIKV infected (< 6 days after symptom onset, positive by PCR test) cohort from the Dominican Republic, the pGOLD IgG/IgA assay can still detect ~ 47% of the ZIKV infection.

3 Assay Kits Available:

1. Simultaneous Zika/Dengue IgG IgA test against ZIKV NS1 and DENV viral antigens

  • Uses low sample volume of ~ 1 µl
  • ~ 2-hour processing time for results
  • IgG and IgA are reported by two different colors of fluorescence.

2. Simultaneous Zika/Dengue IgG IgM test against ZIKV NS1 and DENV viral antigens

  • Uses low sample volume of ~ 1 µl
  • ~ 2-hour processing time for results
  • IgG and IgM are reported by two different colors of fluorescence.

3. Zika/Dengue IgG avidity

  • Uses low sample volume of ~ 1 µl
  • ~ 2-hour processing time for results
  • IgG is reported by 800 nm NIR fluorescence. Each sample will be tested with and without urea treatment.

Format of assays:
pGOLD assay is in a format similar to multi-well microplates widely used in ELISA and can be considered as multiplexed ELISA with very similar assay procedures. Automated washers are readily available and can also be processed manually. Plate reading can be done by MidaScan reader offered by Nirmidas for best results or use existing fluorescence readers.

How the tests can be used:

  • Perform ZIKV/DENV IgG and IgM antibody test; and ZIKV and DENV IgG avidity test.
    • Avidity test can identify if the patient had prior ZIKV or DENV infection.
    • For primary infection without prior flaviviral infection, ZIKV IgG/IgM test can detect ZIKV infection in acute, convalescent and chronic phases.  
  • Perform ZIKV/DENV IgG and IgA antibody test; and ZIKV and DENV IgG avidity test.
    • Avidity test can identify if the patient had prior ZIKV or DENV infection.
    • For patients with prior DENV infection as is the case for adults in dengue endemic regions, ZIKV infection would be considered as secondary infection. ZIKV IgG/IgA test can detect ZIKV infection in acute, convalescent and chronic phase.  Secondary DENV infection can also be detected by pGOLD IgG/IgM and avidity test.

Multiplexed assay kit for a panel of flaviviral and alphaviral infections:
Nirmidas is developing a multiplexed IgG/IgM and IgG/IgA as well as IgG avidity test for ZIKV, DENV, JEV, YFV, WNV, CHIV, BEV and USUTU. The assay will require only 1 micro-liter of serum or whole blood for simultaneous detection of all major flaviviral and alphaviral infections.

Customized assay:
Nirmidas is open to collaborations on customized assays on the pGOLD platform, with any antigens provided by the customers in single-plex or multiplexed formats for diagnosis of infectious and other diseases.

Testing service:
Nirmidas provides testing service for flaviviral infections at a highly affordable price.

Nirmidas Assay Kits are available and customizable. Email us at sales@nirmidas.com or fill in the form below.

For Research Use Only

Download the Multiplexed Flavivirus Product Overview and Catalogue:

 

prenatal infectious disease panel assay Product

Nirmidas MidaScan™ image showing flourescense of multiple biomarkers for each patient sample

Nirmidas MidaScan™ image showing flourescense of multiple biomarkers for each patient sample

Simultaneous screening against TORCH panel: Toxoplasmosis (IgG, IgM), CMV (IgG, IgM), HSV (IgG), Rubella (IgG), HIV (IgG) and Syphilis (IgG)

  • Uses only 2ul serum, plasma or whole blood, or 200ul saliva
  • Kit includes a 56 well plate, assay calibrators, controls, dye-labeled antibodies, and buffer solutions
  • No interference between immobilized proteins (unlike bead-based multiplex assays)
  • Available to purchase. Email us at sales@nirmidas.com or fill in the form below.
  • Compatible with fluorescence-based whole plate reader (using existing instrument or MidaScan/MidaScan-IR scanner provided by Nirmidas)
  • Research Use Only
Nirmidas Assay Kits are available and customizable. Email us at sales@nirmidas.com or fill in the form below.
 

Type 1 diabetes (t1d) multiplexed assay product

Simultaneous screening against T1D autoantibodies

  • Uses only 2ul serum, plasma or whole blood, or 200ul saliva
  • Kit includes a 56 well plate, assay calibrators, controls, dye-labeled antibodies, and buffer solutions
  • No interference between immobilized proteins (unlike bead-based multiplex assays)
  • Available to purchase. Email us at sales@nirmidas.com for more information or fill in the form below.
  • Compatible with fluorescence-based whole plate reader (using existing instrument or MidaScan/MidaScan-IR scanner provided by Nirmidas)
  • Research Use Only
Nirmidas Assay Kits are available and customizable. Email us at sales@nirmidas.com or fill in the form below.
 

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