NIR-II Dye and NIR-I-II Dye

NEAR INFRA-RED dye TECHNOLOGY

Fluorescence-based optical imaging is indispensable to investigating biological systems with high spatial resolution and fast temporal speed.

Imaging in the second near infra-red window (NIR-II), beyond 1000nm, is promising for deep-tissue high resolution optical imaging in vivo:

Dye Nir-II Comparison.png
  • Compared to visible and near infra-red (below ~900nm), the NIR-II window enables reduced scattering of photons traversing through tissues

  • NIR-II optical window imaging can resolve in the sub-5 micron to sub-100 micron range at 3-5 millimeter depth though the intact body and brain of mice.

  • High speed/frame rate imaging allows for blood-flow speed mapping in individual capillary vessels, capable of resolving flow patterns within a single cardiac cycle of mice

  • Non-invasive brain imaging can be achieved without craniotomy

NIR-II DYE: Vessel structure of mouse brain

NIR-II DYE: Vessel structure of mouse brain

Nirmidas’ NIR-II fluorophores are of high quantum yield and can be functionalized by a variety of targeting moieties for molecular imaging and vessel/organ imaging in vitro and in vivo.

NIR-II DYE/PROBE Properties

Nirmidas has developed a series of near infra-red II (NIR-II) dye products suitable for in vivo, ex vivo and in vitro imaging applications. These organic dyes and inorganic probes exhibit the following characteristics.

  • Large Stokes' shift.

  • Excitation (700-1000); emission (1000nm-1800nm).

  • High quantum yield/brightness.

  • Low toxicity to cells in vitro and to small animals in vivo.

  • Blood circulation halftimes ~ 1-5 h, tunable.

  • Rapid excretion from body.

  • Can be conjugated to antibodies, peptides, aptamers and other ligands for molecular imaging and vessel/organ imaging in vitro and in vivo.

NIR Dye Applications

  • Fluorescence imaging/spectroscopy in the NIR-I window (800-1400nm)

  • Fluorescence imaging/spectroscopy in the NIR-II (SWIR) window (1000-1700nm)

  • Molecular imaging of specific biological targets in vivo and ex vivo.

  • Wide field, confocal and light sheet microscopy in NIR-II.

  • Intra-operative imaging

  • Image guided procedures

  • Photoacoustic imaging

  • Two photon imaging in NIR-II

  • Imaging, phototherapy and drug delivery; nanomedicine and theranostics.

Deep-tissue in vivo fluorescence imaging with various animal models for research and development in the research areas of:

  • Cancer

  • Brain/neuroscience

  • Cardiovascular diseases

  • Immune system

Research Use Only

Nirmidas NIR Dye is available to purchase today. Email us at info@nirmidas.com or fill in the form below.


Dye/PROBE LIST

Research Use Only

1)      NIR-II dye. IR-E1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye.

  • Excitation: 650-900 nm. Emission: 900-1400 nm with peak ~ 1050 nm.

  • Brightness 3 out of 5.

  • Functional group: azide, NH2 or COOH. PEG containing, aqueous soluble.

  • In vivo circulation time < 1 hour. Liver excretion.

 

 2)      NIR-II dye. IR-T1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye

  • Excitation: 650-900 nm. Emission: 900-1400 nm with peak ~ 1050 nm.

  • Brightness 3 out of 5.

  • Functional group: N3 azide. PEG containing, aqueous soluble.

  • In vivo circulation time < 1 hour. Liver excretion.

 

 3)      NIR-II dye. np-IR-m1050. Macromolecular, DLS size ~ 15 nm.

  • Excitation: 650-900 nm. Emission: 900-1400 nm with peak ~ 1050 nm.

  • Functional group: NH2. PEGylated, aqueous soluble.

  • Brightness 5 out of 5. Circulation time ~ 3 hours. Liver excretion.

 

 4)      NIR-IIb probe. IR-iNP. Inorganic quantum dot QD based. DLS size ~ 20 nm.

  • Excitation: any wavelength shorter than emission wavelength. Emission peak ~ 1600 nm.

  • Brightness 5 out of 5.

  • Functional group: NH2. PEGylated, aqueous soluble.

  • In vivo circulation time ~ 5 hours. Liver excretion.

* Reference:

“Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging”, Mingxi Zhang, et al., PNAS June 26, 2018 115 (26) 6590-6595;  https://doi.org/10.1073/pnas.1806153115

 

5)      NIR-II probe. HIPCO-CNT biocompatible aqueous suspension. Single-walled carbon nanotube based probe.

  • Single-walled carbon nanotubes (SWNT). Diameter ~ 1 nm. Length ~ 200-300 nm.

  • Brightness 3 out of 5.

  • Excitation: any wavelength in 650-1000 nm. Emission ~ 1000-1400 nm.

  • Functional group NH2. PEGylated, aqueous soluble.

  • In vivo circulation time ~ 3 hours. Liver excretion.

 

6)      NIR-I-II dye. IR-12N. IR-12N3 dye. Molecular MW: ~ 1,000 D.

  • Excitation: 700-850 nm. Emission: 800-1400 nm. Peak ~ 800-850 nm.

  • Brightness 5 out of 5. More stable and brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRdye800 in vivo*.

  • Functional group: N3 azide group. Aqueous soluble. A variant of this dye with NHS group is available upon request.

  • In vivo circulation time < 1 hour. Rapid liver excretion.

* Reference:

Repurposing Cyanine NIR‐I Dyes Accelerates Clinical Translation of Near‐Infrared‐II (NIR‐II) Bioimaging”, S Zhu et al., Adv. Mater; 2018. p. 10. 1002/adma.201802546. https://doi.org/10.1002/adma.201802546

 

Near-Infrared-II (NIR-II) Bioimaging via Off-Peak NIR-I Fluorescence Emission”, S. Zhu et al.,

Theranostics. 2018; 8(15): 4141–4151. doi: 10.7150/thno.27995. http://www.thno.org/v08p4141.htm

 

7)      NIR-I-II dye. IR-12Z-NHS. Molecular MW: ~ 1,000 D.

  • Excitation: 700-850 nm. Emission: 800-1400 nm. Peak ~ 800-850 nm.

  • Brightness 5 out of 5. Brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRdye800 in vivo.

  • Functional group: NHS group. Aqueous soluble. A variant with N3 group is available, IR-12Z-N3, a modified version of IR-12N3 with improved photo stability.

  • In vivo circulation time < 1 hour. Rapid liver excretion.

 

 8)      NIR-I-II probe. np-IR-12. Macromolecular size by DLS ~ 15 nm.

  • Excitation: 780-820 nm. Emission: 800-1400 nm with peak ~ 800-850 nm.

  • Brightness 5 out of 5. High molar extinction coefficient. Bright up to 1300 nm.

  • Functional group NH2 or others. PEGylated, aqueous soluble.

  • In vivo circulation time ~ 3 hour. Liver excretion.

 

9)        NIR-IIb (1500-1700 nm) probe. IR-DCNP. 

  • Rare-earth down-conversion nanoparticle. PEG-NH2 functionalized, aqueous soluble. For CW and lifetime imaging in ~ 1600 nm NIR-IIb under excitation < 1000 nm, please inquire.

 

10)      NIR-I probe. np-NIR1-UV,  ultraviolet excitable. Macromolecular size by DLS ~ 15 nm.

  • Excitation: UV-700 nm. Emission: 800-1100 nm with peak ~ 900 nm.

  • Brightness 5 out of 5.

  • Functional group NH2. PEGylated, aqueous soluble.

  • In vivo circulation time ~ 3 hour. Liver excretion.

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Publications

"A Small-molecule dye for NIR-II imaging" Nature Materials, 15, 235-242, 2016.