NIR-II Dye

NEAR-INFRARED dye TECHNOLOGY

Fluorescence-based optical imaging is indispensable to investigating biological systems with high spatial resolution and fast temporal speed.

Imaging in the second near-infrared window (NIR-II), beyond 1000nm, is promising for deep-tissue high resolution optical imaging in vivo:

Dye Nir-II Comparison.png
  • Compared to visible and near-infrared (below ~900nm), the NIR-II window enables reduced scattering of photons traversing through tissues
  • NIR-II optical window imaging can resolve in the sub-5 micron to sub-100 micron range at 3-5 millimeter depth though the intact body and brain of mice.
  • High speed/frame rate imaging allows for blood-flow speed mapping in individual capillary vessels, capable of resolving flow patterns within a single cardiac cycle of mice
  • Non-invasive brain imaging can be achieved without craniotomy

Nirmidas’ NIR-II fluorophores are of high quantum yield and can be functionalized by a variety of targeting moieties for molecular imaging and vessel/organ imaging in vitro and in vivo.

NIR-II DYE Product

Nirmidas researchers have developed a near-infrared II (NIR-II) dye product (IR-E1050) with high quantum yield and high biocompatibility (renal excretion).

  • Large Stokes' shift
  • Excitation ( 785nm or 808nm); emission (900nm-1300nm)
  • High quantum yield
  • Low Toxicity
  • Rapid excretion from body
  • Functionalizable with a variety of targeting groups for molecular imaging and vessel/organ imaging in vitro and in vivo.
 

NIR-II dye product Series developed by Nirmidas

1. IR-E1050.

NIR-II DYE: Vessel structure of mouse brain

NIR-II DYE: Vessel structure of mouse brain

  • Molecular weight: ~ 3,000. Biliary excretion in vivo.
  • Soluble in aqueous solutions, common buffers, serum and blood
  • Excitation: 808 nm
  • Emission: 900-1400nm, peak ~ 1050nm
  • Quantum yield 5X of SWNTs 

Download Dye IR-E1050 Near-Infrared-II Dye Information sheet (pdf)

2. IR-E1050-COOH.

  • Molecular weight: ~ 3,000; contains COOH group for conjugation to antibodies (protocol available). Biliary excretion in vivo.
  • Soluble in aqueous solutions, common buffers, serum and blood
  • Excitation: 808 nm
  • Emission: 900-1400nm, peak ~ 1050nm
  • Quantum yield 6X of SWNTs

3. IR-T1050.

  • Molecular weight: ~ 2,000. Renal excretion in vivo.
  • Soluble in aqueous solutions, common buffers, serum and blood
  • Excitation: 808 nm
  • Emission: 900-1400nm, peak ~ 1050nm
  • Quantum yield 4X of SWNTs 

3. IR-m1050.

  • A macromolecule based fluorophore. Biliary excretion in vivo.
  • Soluble in aqueous solutions, common buffers, serum and blood
  • Excitation: 808 nm
  • Emission: 900-1400nm, peak ~ 1050nm
  • Ultra-high quantum yield ~ 40-50 X of SWNTs. Allows 1-2 ms exposure times for in vivo imaging.

4. IR-NP.

  • A nanoparticle based fluorophore. Biliary excretion in vivo.
  • Soluble in aqueous solutions, common buffers, serum and blood
  • Excitation: 808 nm
  • Emission: 1400-1700 nm, peak ~ 1600 nm. Huge Stoke's shift.
  • Ultra-high quantum yield ~ 40-50 X of SWNTs. 1-2 ms exposure times possible for in vivo imaging.
 

Applications

  • Fluorescence imaging/spectroscopy in the NIR-II window (1000-1700nm)
  • Photoacoustic imaging
  • Flourescence molecular imaging
  • Small animal imaging
  • Intra-operative imaging
  • Contrast agents
  • Flourescence dye
  • Image guided procedures

The first commercial NIR-II dye has been used for deep-tissue in vivo fluorescence imaging with various animal models related to:

  • cancer
  • brain
  • cardiovascular diseases
  • vascular imaging
Nirmidas NIR-II Dye is available to purchase today. Email us at sales@nirmidas.com or fill in the form below.

Research Use Only

 

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    Publications

    "A Small-molecule dye for NIR-II imaging" Nature Materials, 15, 235-242, 2016.