NIR-II Dye and NIR-I-II Dye

NEAR INFRA-RED DYE TECHNOLOGY

Fluorescence-based optical imaging is indispensable to investigating biological systems with high spatial resolution and fast temporal speed. Combined with Nirmidas DeepVision^TM NIR-II fluorescence imaging instrument and KingsVision^TM dual modality fluorescence and bioluminescence imaging instrument, our cutting-edge infrared dyes will empower research for vasculature, tumor, brain, lymphatic/lymph node imaging and molecular imaging. 

Imaging in the second near infra-red window (NIR-II), beyond 1000 nm, is promising for deep-tissue high resolution optical imaging in vivo:

Compared to visible and near infra-red (below ~900 nm), the NIR-II window enables reduced scattering of photons traversing through tissues NIR-II optical window imaging can resolve micron scale features at 3-5 millimeter depth through the intact body and brain of mice.   High speed/frame rate imaging allows for blood-flow speed mapping in individual capillary vessels, capable of resolving flow patterns within a single cardiac cycle of mice Non-invasive brain imaging can be achieved without craniotomy

Imaging of mouse brain vasculatures using quantum dots (IR-iNP)

Nirmidas’ NIR-II fluorophores are of high quantum yield and can be functionalized by a variety of targeting moieties for molecular imaging and vessel/organ imaging in vitro and in vivo.

NIR-II DYE/PROBE PROPERTIES

Nirmidas has developed a series of near infra-red II (NIR-II) dye products suitable for in vivo, ex vivo and in vitro imaging applications. These organic dyes and inorganic probes exhibit the following characteristics.

• Large Stokes' shift.

• Excitation (700 nm-1000 nm); emission (1000 nm-1800 nm).

• High quantum yield/brightness.

• Low toxicity to cells in vitro and to small animals in vivo.

• Blood circulation halftimes ~ 1-5 hours, tunable.

• Rapid excretion from body.

• Can be conjugated to antibodies, peptides, aptamers and other ligands for molecular imaging and vessel/organ imaging in vitro and in vivo.

NIR DYE APPLICATIONS

• Fluorescence imaging/spectroscopy in the NIR-I window (800 nm-1400 nm)

• Fluorescence imaging/spectroscopy in the NIR-II (SWIR) window (1000 nm-1700 nm)

• Molecular imaging of specific biological targets in vivo and ex vivo.

• Wide field, confocal and light sheet microscopy in NIR-II.

• Intra-operative imaging

• Image guided procedures

• Photoacoustic imaging

• Two photon imaging in NIR-II

• Imaging, phototherapy and drug delivery; nanomedicine and theranostics.

Deep-tissue in vivo fluorescence imaging with various animal models for research and development in the research areas of:

• Cancer

• Brain/neuroscience

• Cardiovascular diseases

• Immune system

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DYE/PROBE LIST

Research Use Only

1)      NIR-II dye. IR-E1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye. 

        (Product No. NBDY-0015)

• Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

• Brightness 3 out of 5. 

• Functional group: azide, NH2 or COOH. PEG containing, aqueous soluble. 

• In vivo circulation time < 1 hour. Liver excretion.

• Spectra:

  
  

  

  
  

 2)      NIR-II dye. IR-T1050. Molecular MW: ~ 3,000 D. Donor-Acceptor-Donor dye.

          (Product No. NBDY-0020)

• Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

• Brightness 3 out of 5.

• Functional group: N3 azide. PEG containing, aqueous soluble. 

• In vivo circulation time < 1 hour. Renal excretion.

• Spectra:

  
  

  

  
  

 3)      NIR-II dye. np-IR-m1050. Macromolecular, DLS size ~ 15 nm. 

          (Product No. NBDY-0022)

• Excitation: 650 nm-900 nm. Emission: 900 nm-1400 nm with peak ~ 1050 nm. 

• Functional group: NH2. PEGylated, aqueous soluble. 

• Brightness 5 out of 5. Circulation time ~ 3 hours. Liver excretion.

• Spectra:

  
  

  
  

  

* Reference: 

“A bright organic NIR-II nanofluorophore for three-dimensional imaging into biological tissues”, Hao Wan, et al., Nature Communications March 21, 2018 1171(2018);  https://www.nature.com/articles/s41467-018-03505-4

           

 4)      NIR-IIb probe. IR-iNP. Inorganic PbS/CdS core-shell quantum dots (QD), in PBS. DLS size ~ 20 nm. 

          (Product No. NBDY-0018A) 

• Excitation: any wavelength shorter than emission wavelength. Emission peak ~ 1600 nm. 

• Brightness 5 out of 5.

• Functional group: NH2. PEGylated, aqueous soluble. 

• In vivo circulation time ~ 5 hours. Liver excretion.

  
  

     NIR-IIb probe. IR-iNP. Inorganic PbS/CdS core-shell quantum dots (QD), in Organic Solvent. DLS size ~ 20 nm. 

     (Product No. NBDY-0018B). 

• Default solvent: Chloroform. If you prefer nanoparticles in other organic solvent (Toluene, DCM etc.), please contact us by filling the inquiry form. 

* Reference: 

“Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging”, Mingxi Zhang, et al., PNAS June 26, 2018 115 (26) 6590-6595;  https://doi.org/10.1073/pnas.1806153115

• Spectra:

  

  
  

  
  

5)      NIR-II probe. HIPCO-CNT biocompatible aqueous suspension. Single-walled carbon nanotube based probe.

         (Product No. NBDY-0028)

• Single-walled carbon nanotubes (SWNT). Diameter ~ 1 nm. Length ~ 200 nm-300 nm. 

• Brightness 3 out of 5.

• An ideal quantum yield reference standard. Non-photobleaching. Absolute QY~0.03% measured by integrated sphere for >1000 nm emission

• Excitation: any wavelength in 650 nm-1000 nm. Emission ~ 1000 nm-1400 nm. 

• Functional group NH2. PEGylated, aqueous soluble. 

• In vivo circulation time ~ 3 hours. Liver excretion.

6)      NIR-I-II dye. IR-12N. IR-12N3 dye. Molecular MW: ~ 1,000 D. 

         (Product No. NBDY-0002)

• Excitation: 700 nm-850 nm. Emission: 800 nm-1400 nm. Peak ~ 800 nm-850 nm. 

• Brightness 5 out of 5. More stable and brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRDye 800CW in vivo*.

• Functional group: N3 azide group. Aqueous soluble. Refer to NBDY-0014 for a variant of this dye with NHS group is available upon request.

• In vivo circulation time < 1 hour. Rapid liver excretion.

* Reference: 

1) “Repurposing Cyanine NIR‐I Dyes Accelerates Clinical Translation of Near‐Infrared‐II (NIR‐II) Bioimaging”, S Zhu et al., Adv. Mater; 2018. p. 10. 1002/adma.201802546. https://doi.org/10.1002/adma.201802546

 

2) “Near-Infrared-II (NIR-II) Bioimaging via Off-Peak NIR-I Fluorescence Emission”, S. Zhu et al.,

Theranostics. 2018; 8(15): 4141–4151. doi: 10.7150/thno.27995. http://www.thno.org/v08p4141.htm

• Spectra:

  
  

  
  

  
  

7)      NIR-I-II dye. IR-12-NHS. Molecular MW: ~ 1,000 D. 

         (Product No. NBDY-0014)

• Excitation: 700 nm-850 nm. Emission: 800-1400 nm. Peak ~ 800 nm-850 nm. 

• Brightness 5 out of 5. Brighter than ICG. High molar extinction coefficient. Replacement for ICG, IRDye 800CW in vivo. 

• Functional group: NHS group. Aqueous soluble. 

• In vivo circulation time < 1 hour. Rapid liver excretion.

 8)      NIR-I-II probe. np-IR-12. Macromolecular size by DLS ~ 15 nm. 

          (Product No. NBDY-0026)

• Excitation: 780 nm-820 nm. Emission: 800 nm-1400 nm with peak ~ 800 nm-850 nm. 

• Brightness 5 out of 5. High molar extinction coefficient. Bright up to 1300 nm.

• Functional group NH2 or others. PEGylated, aqueous soluble. 

• In vivo circulation time ~ 3 hours. Liver excretion.

9)        NIR-IIb (1500-1700 nm) probe. IR-DCNP. Er based rare-earth down-conversion nanoparticle. 

           (Product No. NBDY-0029A)

• Excitation: 980 nm. Emission: 1540 nm.

• Brightness: 5 out of 5. 

• Functional group: PEG-NH2 functionalized, aqueous soluble.

• In vivo circulation time ~ 5 hours. Liver excretion. 

• For Continuous wavelength and lifetime imaging. 

• Nanoparticles soluble in organic solvents without transferred to aqueous phase are available upon request. Inquires please specify IR-DCNP-O (Product No. NBDY-0029B).

  
  

* Reference: 

“In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles”, Y. Zhong et al., Nature Biotechnology; 2019 Nov; 37 (11): 1322-1331. doi: 10.1038/s41587-019-0262-4. https://www.nature.com/articles/s41587-019-0262-4

• Spectra:

  

  
  

10)      NIR-I probe. np-NIR1-UV,  ultraviolet excitable. Macromolecular size by DLS ~ 15 nm.

           (Product No. NBDY-0013)

• Excitation: UV-700 nm. Emission: 800 nm-1100 nm with peak ~ 900 nm. 

• Brightness 5 out of 5.

• Functional group NH2. PEGylated, aqueous soluble. 

• In vivo circulation time ~ 3 hours. Liver excretion.

11)      NIR-I-II probe. AuMC (Gold Molecular Clusters)

           (Product No. NBDY-0039)

• Containing ~ 25 gold atoms with glutathione (GSH) hydrophilic surface ligands. Cluster size <~ 3 nm 

• Excitation: any wavelength shorter than ~900  nm. Emission range ~ 850-1350 nm
  

• Brightness 4 out of 5.

• Functional group: COOH and NH2. Aqueous soluble. 

• In vivo circulation time < 1 hour. Renal excretion.

*Reference:

‘Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models’, Ani Baghdasaryan, Feifei Wang, Fuqiang Ren, Zhuoran Ma, Jiachen Li, Xueting Zhou, Lilit Grigoryan, Chun Xu, Hongjie Dai, 2022/9/24, Nature communications, Volume 13, Issue 1, Pages 1-11. https://pubmed.ncbi.nlm.nih.gov/36153336/

• Spectra:

        

Nirmidas NIR Dye is available to purchase today. Email us at info@nirmidas.com or fill in the form below.

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